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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
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Fecha : |
18/09/2014 |
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Actualizado : |
25/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; REBUFFO, M.; DALLA RIZZA, M.; FOLLE, G.; SANTIÑAQUE, F.; BORSANI, O.; MONZA, J. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Generation and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Crop Science, 2012, v. 52, no. 4, p. 1572-1582. |
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ISSN : |
0011-183X |
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DOI : |
10.2135/cropsci2011.07.0374 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published July, 2012. |
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Contenido : |
ABSTRACT.
A method is described for obtaining interspecific hybrids between commercial lines of Lotus uliginosus Schkuhr and Lotus corniculatus L. Hybridization was possible between these species using embryo rescue. Two strategies were used to confirm 40 F1 hybrids, that is, flow cytometry and microsatellite simple sequence repeats (SSRs). The L. uliginosus and L. corniculatus parents have significantly different genome size (2.5 ± 0.02 and 2.22 ± 0.02 pg, respectively) and the offspring from the cross had intermediate values. Two SSR primers were polymorphic between the genotypes tested, TM1150 and EH380069. Maternal effect was detected in L. uliginosus genotypes, as determined by greater frequency of F1 hybrids. Recombinant traits were observed in the F1 hybrid progeny. Ninety F1 hybrids resulting from L. uliginosus × L. corniculatus crosses showed different levels of fertility in the polycross; <3% F1 plants produced more than 1000 seeds without embryo rescue while 10% of plants did not flower the first year. The F1 hybrid plants grown under greenhouse conditions exhibited shoot phenotypes similar to L. uliginosus (maternal) whereas F2 phenotypes were similar to L. corniculatus (paternal) in field trials. Rhizome presence was observed in 60% of F2 hybrid plants. The diameters of the root crowns of these plants, however, were similar to that of L. corniculatus.
© Crop Science Society of America. |
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Palabras claves : |
LEGUMINOSAS FORRAJERAS; MARCADORES MOLECULARES. |
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Thesagro : |
CULTIVOS; PLANTAS FORRAJERAS. |
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Asunto categoría : |
--
F01 Cultivo |
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Marc : |
LEADER 02262naa a2200277 a 4500
001 1050609
005 2019-11-25
008 2012 bl uuuu u00u1 u #d
022 $a0011-183X
024 7 $a10.2135/cropsci2011.07.0374$2DOI
100 1 $aCASTILLO, A.
245 $aGeneration and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus.$h[electronic resource]
260 $c2012
500 $aArticle history: Published July, 2012.
520 $aABSTRACT. A method is described for obtaining interspecific hybrids between commercial lines of Lotus uliginosus Schkuhr and Lotus corniculatus L. Hybridization was possible between these species using embryo rescue. Two strategies were used to confirm 40 F1 hybrids, that is, flow cytometry and microsatellite simple sequence repeats (SSRs). The L. uliginosus and L. corniculatus parents have significantly different genome size (2.5 ± 0.02 and 2.22 ± 0.02 pg, respectively) and the offspring from the cross had intermediate values. Two SSR primers were polymorphic between the genotypes tested, TM1150 and EH380069. Maternal effect was detected in L. uliginosus genotypes, as determined by greater frequency of F1 hybrids. Recombinant traits were observed in the F1 hybrid progeny. Ninety F1 hybrids resulting from L. uliginosus × L. corniculatus crosses showed different levels of fertility in the polycross; <3% F1 plants produced more than 1000 seeds without embryo rescue while 10% of plants did not flower the first year. The F1 hybrid plants grown under greenhouse conditions exhibited shoot phenotypes similar to L. uliginosus (maternal) whereas F2 phenotypes were similar to L. corniculatus (paternal) in field trials. Rhizome presence was observed in 60% of F2 hybrid plants. The diameters of the root crowns of these plants, however, were similar to that of L. corniculatus. © Crop Science Society of America.
650 $aCULTIVOS
650 $aPLANTAS FORRAJERAS
653 $aLEGUMINOSAS FORRAJERAS
653 $aMARCADORES MOLECULARES
700 1 $aREBUFFO, M.
700 1 $aDALLA RIZZA, M.
700 1 $aFOLLE, G.
700 1 $aSANTIÑAQUE, F.
700 1 $aBORSANI, O.
700 1 $aMONZA, J.
773 $tCrop Science, 2012$gv. 52, no. 4, p. 1572-1582.
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INIA Las Brujas (LB) |
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| Registros recuperados : 7 | |
| 2. |  | SANTIÑAQUE, F. Relaciones agua-planta en pasturas In: MORON, A.; MARTINO, D.; SAWCHIK, J. (Eds.). Manejo y fertilidad de suelos . Montevideo (Uruguay): INIA, 1996. p. 125-128 (INIA Serie Técnica ; 76) Información presentada en el II Curso de Actualización Técnica Manejo y Fertilidad de Suelos, 1995 set 4-8, La Estanzuela| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 5. | | CASTILLO, A.; REBUFFO, M.; DALLA RIZZA, M.; FOLLE, G.; SANTIÑAQUE, F.; BORSANI, O.; MONZA, J. Generation and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus. Crop Science, 2012, v. 52, no. 4, p. 1572-1582. Article history: Published July, 2012.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
|  |
| 6. | | CASTILLO, A.; LOPEZ, V.; TAVARES, E.; SANTIÑAQUE, F.; DALLA RIZZA, M. Polyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs]. Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. Agrociencia Uruguay 2020, v. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413 9 p. Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : -- - -- |
| Biblioteca(s): INIA Treinta y Tres. |
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| 7. | | CASTILLO, A.; REBUFFO, M.; DALLA RIZZA, M.; GARCIA, C.; FOLLE, G.; SANTIÑAQUE, F.; BORSANI, O.; MONZA, J. Generación y caracterización de híbridos interespecíficos de L. uliginosus x L. corniculatus ln: Jornada técnica, VI Jornada de agrobiotecnología. INIA Las Brujas, 20 de octubre de 2012 Conocimiento intensivo para el sector productivo: situación actual y perspectivas. Canelones (UY): INIA, 2012. 15-16 (Serie Actividades de Difusión; 698)| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 7 | |
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